white as well as 'near' white pixels will be transparent. On the other hand a non-zero value say N will make transparent all pixels with RGB values where the R G and B components have values from (255-N) to 255 i.e. A threshold value of 0 will make transparent all pixels with RGB values where the R G and B components are all exactly 255 i.e. If your logo does not have this property you can optionally have its background be made transparent provided it is white (or 'near' white) by entering a transparency threshold value.
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For a lot of us, especially in the early part of 2021, it meant moping around, curtain twitching to get a look at a passing dog just to feel something, and screaming “I’m sick of making fucking sourdough” in manner of Gemma Collins on Celebrity Big Brother. It's a grim tale of sin and retribution, brought to life as one of the most intense physical experiences you could possibly have while taking a government-sanctioned stroll around the block with your Air Pods. "Do you wanna be in hell with me?" she asks, referencing an 18th century legend of the Pennsylvania Dutch, about an iron master who throws his dogs into the furnace for underperforming on a hunt only to see them return and drag him to hell. Reverberating with heavy piano and rumbling drums, "Pennsylvania Furnace" sounds like the fear of God itself – his judgement, his abandonment. Her fourth album, SINNER GET READY, takes that trauma and places it within the religious history and generally austere vibe of rural Pennsylvania, where Hayter lived until recently. Themes of violence against women, the body, and revenge have run through each of her projects, taking the form of all kinds of sounds from operatic splendour to abrasive noise – as close to corporeal as it's possible for music to get – in an effort to articulate trauma ("lingua ignota" Latin for “unknown language”). Lingua Ignota's Kristin Hayter has long been rummaging through the landfill of despair. ![]() ![]() Not so with this “One Step Closer” reanimation, which takes a song that genuinely seems to express something about how we experience our relentlessly stressful times, and makes it sound like the way we experience them like the dark heart of the algorithm. ![]() So much of the culture that surrounds us centres on remaking or rebooting for its own sake, often producing results on the spectrum between “underwhelming” and “actually brain-numbing”. Dylan Brady and Laura Les continued their run of fascinating and irresistible post-everything hijinks into this year, and while Brady’s new version of Rebecca Black’s “Friday” featuring Dorian Electra, Big Freedia and 3OH!3 was almost the best thing the gecs-verse produced this year, this new take on “One Step Closer” has it beat in terms of sheer mayhem. There couldn’t have been a more fitting anthem for 2021 than a rework of Linkin Park’s furious declaration of hopelessness “One Step Closer”, by popular culture’s premier chaos goblins 100 gecs. By comparison, Pokémon GO took in approximately $1.6 million in those markets during the same period of time. ![]() To date, Wizards Unite has grossed an estimated $72,000 in Australia and New Zealand ahead of releasing in the U.S. Pokémon e os nomes dos personagens Pokémon são marcas da Nintendo. We'll be closely monitoring its performance throughout the Harry Potter: Wizards Unite launch period, which will overlap with Pokémon GO's third launch anniversary on July 6. O Pokémon Go pode ser jogado em inglês, francês, alemão, italiano, russo, japonês, espanhol, chinês tradicional, coreano, tailandês, português do Brasil, turco e indonésio. Select the raid boss you want to join and it will match automatically. 11 among all apps for revenue last month. Raid Finder You can join or invite remote raids around the world anytime, anywhere. Last year, Japan had contributed the most revenue by this point at 33 percent of all spending in the title. GO When Pokmon GO was first developed, Niantic used a map from a previous AR game called Ingress, which was built from a combination of. Panpour will be located in the Americas and Greenland. Pansear will be located in Europe, the Middle East, Africa and India. The 2019 event, unlike the preceding ones, were to be held in three separate cities Chicago (Grant Park. Pansage will be located in the Asia-Pacific region. has led spending in 2019 to date, accounting for about 35 percent of the total, with Japan representing 31 percent of revenue. A third Go Fest event was announced on 4 April 2019. ![]() ![]() The title's global player base has spent an estimated $368 million so far in 2019, which is about 21 percent more than the $304 million it generated during the same period in 2018. The publisher's 2016 hit Pokémon GO has generated $2.6 billion in player spending to date, according to Sensor Tower Store Intelligence estimates, with $55 million of that added last month alone. Niantic's third location-based AR mobile game, Harry Potter: Wizards Unite, launches in the United States and United Kingdom on Friday, and, by all accounts, has a lot to live up to. We also explored a complication to the above hypotheses: harmonics. Our primary purpose was to test these two hypotheses. In this case, the wing acts as a stiff reactive element with respect to the fundamental wingbeat frequency. The alternative stiff element hypothesis is that the resonance frequency of the first longitudinal mode of the wings is far above the wingbeat frequency. Moreover, the type of wing flexion that is known to have beneficial aerodynamic effects is often perpendicular to the long axis of the wing, in the chordwise direction ( Mistick et al., 2016 Mountcastle and Combes, 2013). However, resonance can also be detrimental to flight, because driving a system at resonance potentially hinders control, especially the ability to modulate frequency away from the resonance frequency, which insects may need to maneuver. Thus, one possibility, the resonance hypothesis, holds that the wing resonances are matched to the wingbeat frequency. For example, it could increase stroke amplitude (as measured at the tip of the wing) relative to the excursion of this same angle as measured at the base of the wing, thereby mechanically amplifying the motion of the wing while permitting the muscles driving the wing to operate with lower strain. Flexion in this axis may be beneficial in certain scenarios. ![]() The lowest resonance frequency of the wings will be the first longitudinal mode of the long axis ( Fletcher, 1992). little energy is lost to internal damping). If you would like to receive future posts by email, please subscribe above.Driving a system near resonance can be beneficial because it is advantageous for energy transfer (i.e. ![]() If you have found this post interesting or helpful, please comment below and like/share on Facebook. The wing morphology analysis I carried out last year showed that the samples of bees taken from my colonies, ranged from between 80% to 100% of the profile of Apis mellifera mellifera. The results of the analysis are shown in the small windows at the bottom of the screen and can be printed out in a report. The software shows how these coordinates fit with the three main measures used for wing morphology analysis, against the normal ranges of different strains of honey bees. The next stage is to go into the analysis part of the CBeeWing software and to look at the coordinates produced. The three main measures that are used for wing morphology are cubital index, discoidal shift and Hantel index. It takes some time to learn how to do this but after a couple of samples the process speeds up so you can do a sample of 30 wings in about 15 minutes. jpg file from the scanner is opened in CBeeWing and the key points of the veins on each of the wings is plotted using the system. I tried free software for the analysis of honey bee wings but decided to buy the licence for CBeeWing from Cybis and to download it. This slide is then scanned at the highest resolution on my all-in-one printer to produce a. A glass microscope slide is placed over the wings and the sellotape is cut to size and labelled. These wings are placed carefully on a piece of sticky side up sellotape. The technique I use for wing morphology involves removing the right wing from a sample of around 30 bees from a colony. I decided then that I wanted all future queens to come from this colony. They were a pleasure to handle and they always appeared to be purposeful and effective in whatever they were doing. ![]() The rest of the season they flourished, they were very thrifty on stores during the winter and appeared to get going quickly as they went into the spring. None of my other bees did this or had this attitude! They seemed considered and organised and I’d swear they were watching my every move. In the evening they filled the entrance and looked out, so all you could see was a sea of little bee faces starring out of the small round hole. They had less colour on their abdomen and they behaved differently. I had five colonies at the time, but these bees were different. It would have struggled to fill a pint pot. Six years earlier, I caught a small swarm from my garden fence, here in rural Northumberland. Where did I get my native or near-native bees ? Using the software tool CBeeWing, colonies showed results which classified the samples as being between 80%-100% Apis mellifera mellifera. The results were a surprise, as it looked like my bees are native, or at least near-native anyway. In the spring of last year, I decided to investigate how native my bees were, so I conducted wing morphology on all of my colonies, a technique which involves measuring positions of vein intersections on the bee’s main wing. Wing Morphology – How native are my bees? ![]() ![]() Chuang, Cervical cancer: a global health crisis, (2017) 1–9. Jemal, Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries, CA. The identification of proteins and their associated pathways provides a deeper understanding of the underlying molecular mechanisms involved in HPV-associated cervical cancer.Īcknowledgement: The work was funded by the Fundamental Research Grant Scheme (FRGS/1/2019/SKK13/USM/01/1) from the Ministry of Education, Malaysia. The key pathways involved were viral carcinogenesis and necroptosis, reflecting their role in cell proliferation, migration, and metastasis. The main molecular function was the binding process, and the top biological processes were chromatin silencing for SIL compared to the normal cervix and nucleosome assembly for the SCC compared to SIL group. Interestingly, the quantification results showed an upward trend for the up-regulated proteins and a downward trend for the down-regulated proteins in the progression from normal to SIL and SCC. The predominant protein found was histone. We identified a total of 3597 proteins with 589, 550, and 1570 proteins unique to the normal cervix, SIL, and SCC groups, respectively, while 332 proteins were similar across all three groups. Bioinformatics analyses were carried out using DAVID, ConsensusPathDB, and STRING. The samples were trypsin digestion and subjected to high throughput liquid chromatography-electrospray ionization-tandem mass spectrometry (LC/ESI-MS/MS) and trapped ion mobility time-of-flight-mass spectrometry (timsTOF-MS), followed by quantification with MaxQuant and Perseus software. The pathological regions of interest in the cervical squamous epithelium were micro-dissected from formalin-fixed paraffin-embedded (FFPE) tissue sections of six normal cervix cases, five HPV-associated squamous intraepithelial lesion (SIL), and six squamous cell carcinomas (SCC). ![]() This study aims to elucidate the differentially expressed proteins and offer insights into the cellular processes and pathways involved in the development of cervical cancer. However, the proteome alterations involved in cervical carcinogenesis are not extensively studied. Tissue-based proteomics is an evolving tool used in cancer research to characterize the pathophysiology of disease. Gaayathri Kumarasamy 1, Mohd Nazri Ismail 1, 2, Sharifah Emilia Tuan Sharif 3, Christopher Desire 4, Parul Mittal 4, Peter Hoffmann 4 and Gurjeet Kaur 1 1Institute for Research in Molecular Medicine (INFORMM), Universiti Sains Malaysia, 11800 Minden, Pulau Pinang, Malaysia 2Analytical Biochemistry Research Centre (ABrC), Universiti Sains Malaysia, 11900 Bayan Lepas, Pulau Pinang, Malaysia 3Department of Pathology, School of Medical Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia 4Clinical Health Sciences, University of South Australia, City West Campus, Adelaide, South Australia, 5000, Australia Correspondence: Gurjeet Kaur The International Conference on Molecular Diagnostics & Biomarker Discovery 2022 (MDBD 2022) ![]() Orange – When dreaming about the color orange the bible describes this color as being of fire. 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